EIP Project Database

Find EIP-AGRI projects in Germany

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Multiserology via Microarray: A cost-effective diagnostic tool for continuous improvement of animal health and food safety in pig farms

Authority

Niedersächsisches Ministerium für Ernährung, Landwirtschaft und Verbraucherschutz

Federal State

Lower Saxony and Bremen

Contact

Calenberger Straße 2
30169 Hannover

Website: External link to the authority

Project Title

Multiserology via Microarray: A cost-effective diagnostic tool for continuous improvement of animal health and food safety in pig farms

Project description

With regard to the occurrence of endemically occurring zoonoses and so-called production diseases in the food chain, the Regulation (EC) No 854/2004 and the EFSA (Scientific Opinion, 2011) demand for serological monitoring programmes. This claim is implemented only for Salmonella in Germany. Therefore, the aim of this study is to establish an information system for zoonotic and production diseases of pigs, which is based on serological tests. This information system should include the interests of all stakeholders along the food chain.  In these investigations a microarray will be deployed as an exemplary, serological test platform. Thus, an already existing microarray prototype should be used for detection of relevant pathogens, and should be integrated in the meat inspection system. Furthermore, the information system will be tested in practical trials with 60 pig farms. In this scenario the priority is to evaluate and to optimize the information flow. Moreover, farm specific intervention measures and control plans for food safety should be developed, and practically tested.

Results focusing on practiceorientation

n order to monitor the occurrence of zoonotic pathogens in pig herds and to improve health management in the barn, the development of new cost-effective diagnostic methods for pig herds is required. As part of the "Multiserology via Microarray" project, a protein microarray-based test was developed for the simultaneous detection of immunoglobulin G antibodies against zoonotic pathogens and respiratory pathogens in pigs. The chip was initially tested in a small reaction tube (ArrayTube) and subsequently in a 96-well compatible format (ArrayStrip). Blood serum and meat juice samples could be used as sample material on both formats, so that samples from the German pig salmonella monitoring programme from the slaughterhouse, for example, could be used on the microarray for the study.

Key words

  • Pest/disease control
  • Food security, safety, quality, processing and nutrion
  • Supply chain, marketing and consumption

Main funding source

Rural development 2014-2020 for Operational Groups (in the sense of Art 56 of Reg.1305/2013)

OG contact information

Stiftung Tierärztliche Hochschule Hannover; Institut für Lebensmittelqualität und –sicherheit
Bischofsholer Damm 15
30173 Hannover

Phone: 0511/8567551

Email: diana.meemken@tiho-hannover.de

Type of OG partners

  • Advisor
  • Researcher
  • Industry
  • Wholesaler

Begin of project

2016

Project status

completed

Total budget applied for in EUR

443,807

Further information

Weitere Informationen finden Sie im Praxisblatt.

Link 1

Practice Sheet

Link 3

NUTS3 CODE

DE929

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